Novel hybrid Technology for Bioseparation

Student of Institute of Chemical Technology, Mumbai Jayeshkumar Sevantilal Mevada won the award for research work of Novel hybrid Technology for Bioseparation. They carried their project work under the guidance of  Prof. Aniruddha B. Pandit.

In Current era, the unaffordability of medicine to rural peoples and pollution of the river are the big issues for the developing countries. A biological approach is an alternative way for the replacement of hazardous chemical processes responsible for water pollution, but the cost is the primary concern for its applicability. The microbial cell is the factory of various biological products located intracellularly or extracellularly. 

Conventional method (High-pressure homogenizer) causes the complete cell disruption which results in the release of all the contaminating materials with the target product of interest. This contaminating materials restrict further purification which leads to problems such as membrane fouling, higher processing times, reduced life of chromatographic column, increase in the steps of purification (10-13 steps) and higher cost of purification (70 to 80 % cost of total production cost).

We have developed novel hybrid technology where controlled cavitation using the hydrodynamic cavitating device was used for the selective recovery of intracellular biomolecules at microbial cell disruption stage (with a pretreatment regimen of acidic as well as alkaline conditions). Synchronizing of pretreatment and cavitating conditions result in selective recovery of biomoleculesfrom the cytoplasmic and periplasmic location of cells with minimal contaminating materials. The developed technology is believed to be novel and a breakthrough in the field of bioseparation. The expensive multi-bioseparation steps can be avoided because of the selective recovery of biomolecules at the initial stage. The uniqueness of this invention when compared with the existing technologies augment its characteristics in selectiveness, energy efficiency, easily scalable, reduced number of steps (from 10-13 to 3-5), low overall cost (3-4 times), 4-5-time reusability of immobilized product. Apart from the aforementioned qualities, the cost reduction by the developed technology can be considered as the big initiative step toward the greener processes and affordable cost biotherapeutics.

The Hon’ble Vice President of India, Shri M. Venkaiah Naidu awarded the Gandhian Young Technological Innovation (GYTI) Award to Jayeshkumar Sevantilal Mevada, Institute of Chemical Technology, Mumbai at the GYTI 2019 Awards function held at Vigyan Bhawan, New Delhi on July 06, 2019.

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