Magnetic-field actuated hybrid nanofiber scaffold and apparatus for 4D tissue engineering

Students of Indian Institute of Technology, Roorkee Uday Kumar Sukumar and Vinay Kumar won the award for research work of Magnetic-field actuated hybrid nanofiber scaffold and apparatus for 4D tissue engineering. They carried their project work under the guidance of Dr P. Gopinath.

The present invention provides a hybrid multi-layered nanofibrous scaffold and a four-dimensional dynamic cell culture apparatus. The pre-existing 3D scaffolds in the market does not address the dynamic culture conditions covered in this technology, therefore, they do not represent the in-vivo conditions as closely as the 4D scaffold proposed in this research work. The market of 4D scaffolds is focussed on the area of therapeutics and diagnostics. 4D scaffolds are expected to reduce the need for animal disease models, as they are versatile in accustoming to different culture conditions, and are more relevant for cell culture studies as compared to 3D scaffolds. Furthermore, the scaffold is biocompatible and operates in non-contact mode and it is precisely controlled across all 3- dimensions. The hybrid scaffold apart from providing the extracellular matrix (ECM)-like scaffold, also meticulously simulates the mechanical stress cycles that cells experience under the in-vivo conditions.

For monitoring cells on a real-time basis under microscopes, the apparatus is provided with sufficient headspace over the tissue culture plate for positioning a microscope lens. The circular slot provided at the base of the apparatus perfectly accommodates cell culture plates and also enables easy transmission of light during microscopic observation without any interference of the apparatus body. Cell plasticity, differentiation and cancer metastasis are determined to a large extent by cell adhesion and surface proteins which are effectively recapitulated in this scaffold. The scaffold serves as a realistic and relevant platform for studying the role of cytoskeletal proteins and focal adhesion proteins in various diseases in the fields of cardiology, dermatology, metabolism, gastroenterology, oncology and orthopaedics.

The Hon’ble Vice President of India, Shri M. Venkaiah Naidu awarded the Gandhian Young Technological Innovation (GYTI) Award to Uday Kumar Sukumar and Vinay Kumar, Indian Institute of Technology, Roorkee at the GYTI 2019 Awards function held at Vigyan Bhawan, New Delhi on July 06, 2019.

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