Point of Care Nano Diagnostic Kit for Brucellosis

Students of Institute of Chemical Technology, Mumbai, Rohit Shivaji Pawar, Swati Suhas Vyas won the award for developing a prototype of Point of Care Nano Diagnostic Kit for Brucellosis. They carried their project work under guidance of Dr. Vandana B. Patravale.

Brucellosis, a neglected global zoonotic infectious disease is transmitted to humans through consumption of unpasteurized dairy products and direct contact with afflicted animals. Presence of bacteria such as Brucella spp. in dairy products is an immense risk to public health. The innovation herein describes the development of immunochromatographic diagnostic kit (ICDT) based on fluorescence silica nanosensors that serves as a promising nanodiagnostic approach for detection of intact Brucella antibodies in nonserological samples eliminating painful blood collection procedures. Point of care immunoassays are rapid as they can quickly screen various samples in relatively shorter duration, are sensitive, specific, offer great advantage in accurate and fast diagnosis of infectious diseases. We have fabricated a point of care rapid diagnostic assay that employs fluorescent, micellar silica nanosensors conjugated with lipopolysaccharides (Indian patent application 3183/MUM/2013) capable of specifically detecting Brucella IgG antibodies in non-serological samples of afflicted animal (milk, urine, saliva) and human samples (urine, saliva).


The developed kit holds its industrial application in agricultural , biotechnological and pharmaceutical arena. We have successfully fabricated and evaluated the aforementioned kit. The developed ICDT provides a rapid, reliable, point-of-care, accurate and reproducible results in accordance with PCR and ELISA results with high sensitivity and specificity.Additionally, well defined antigenic components and surface biomarkers of various disease causing microbes can be broadly incorporated within the purview of this technology for accurate and rapid detection of suspected bovine pathological conditions, and can largely enable rapid field testing that can be implemented in farms and food Industry. Since the developed technology is novel, cost effective yet scalable; it fits “Make In India” initiative to foster innovation, enhance skill development, create intellectual property and generate revenue.

The Hon’ble President of India, Shri Ram Nath Kovind awarded the Gandhian Young Technological Innovation (BIRAC – GYTI) Award to  Rohit Shivaji Pawar, Swati Suhas Vyas, Institute of Chemical Technology, Mumbai at the GYTI 2018 Awards function held at Rashtrapati Bhawan on March 19, 2018.

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