Smartphone-based impedimetric disposable biosensor for detection of cardiac biomarkers

Students of Indian Institute of Technology Bombay, Debasmita Mondal, Sourabh Agrawal won the award for developing a prototype of Smartphone-based impedimetric disposable biosensor for detection of cardiac biomarkers. They carried their project work under guidance of Dr. Soumyo Mukherji.

A smartphone-enabled disposable, label-free, impedimetric biosensor to detect two common cardiac biomarkers, Myoglobin (Myo) and Myeloperoxidase (MPO) is developed. The device consists of a sensor and a module for interfacing with smartphone. The sensor, fabricated on Whatman filter paper, is coated with a conducting polymer, polyaniline (PAni) and immobilized with antibodies specific to each biomarker protein. Non-specific active regions of the sensor are blocked by treatment with bovine serum albumin. Electrodes for AC impedance measurement are deposited using conductive silver paste. Binding of target protein to specific antibody affects the dielectric constant between the electrodes, thus changing the impedance. Myo and MPO in buffer solution are detected from 100 ng/mL – 50 μg/mL within 100 Hz – 1 kHz. Detection limit obtained for these biomarkers spiked in human serum is 500 ng/mL. The developed biosensor uses inexpensive materials and fabrication techniques, detects cardiac biomarkers in clinically relevant concentrations rapidly (~20 minutes), can be disposed in an environment friendly manner, thus making the sensor suitable for diagnostic applications. To make a handheld, portable point-of-care device, a plug and play module for smartphone is developed providing a cost and space effective solution. Prepared module acts as a peripheral to the phone and can be carried in pocket along with headphone. A lowpower and low-cost potentiostat is designed in the peripheral which connects to the audio jack and gets power from the USB port of smartphone. Frequency range of the peripheral is limited to audio range (10 Hz – 10 kHz). Maximum duration each frequency signal can be generated is 5 Seconds. Onetime buying price of a single unit at research level is ~INR 1500 along with a minimal price for cartridge i.e., the sensor.

The Hon’ble President of India, Shri Ram Nath Kovind awarded the Gandhian Young Technological Innovation (BIRAC – GYTI) Award to Debasmita Mondal, Sourabh Agrawal, Indian Institute of Technology Bombay at the GYTI 2018 Awards function held at Rashtrapati Bhawan on March 19, 2018.

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