Genetic Engineering Of Brassica Juncea For Drought Tolerance
Student Team/ Author :
Bedabrata Saha
Guide By :
Prof. Sanjib Kumar Panda
Degree :
Others
College :
Assam University Silchar
Indian mustard [Brassica juncea (L.) Czern & Coss.] is one of the most important oilseed crop worldwide,
yet relatively mild drought and salinity stress significantly reduce its growth and yield. Expression of LEA
(Late Embryogenesis Abundant) proteins was previously shown to alleviate osmotic stress by stabilizing
water status, protecting cytosolic structures and cell through increased membrane and protein stability.
We overexpressed Arabidopsis group 4 LEA protein AtLEA4-1 in mustard, resulting the transgenic lines to
show enhanced drought and salinity tolerance. Southern hybridization showed the copy number of the
gene inserted into the transgenics while, qRT PCR determined the transcript level of the overexpressed
gene. Leaf senescence assay showed better adaptability of the transgenics to both salt and PEG stress, as
revealed by increased chlorophyll content in the transgenic plants when compared to wild type. The
transgenic mustard plants showed better survivability under both drought and salinity stress in soil.
Physiological analysis revealed lower levels of lipid p
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