Novel applications of tannase can be developed .Ethanol production from agro industries are of great importance. Phenols generated from lignin through prior treatment of the agro wastes. Phenols are known to inhibit the hydrolytic activity of cellulase. These phenol compounds can be rapidly degraded by tannase and thus subsequently lead to the increase in the catalytic activity of cellulase. In humans identification of Staphilococcus lugdunensis can be carried out by the tannase gene and their respective enzyme activity and can be employed to prevent the colon cancer. Therapeutic applications of tannase enzyme involve generation of novel molecules, such as prunioside A with anti-inflammatory activity (R Bello-Mendoza et al,2008). Other major uses of tannase are found in the manufacture of laundry detergents in the form of additive and homogenization of the tannins preparation for good grade leather tannins in the leather industry. Tannin is an anti-nutrient that is present in tea, since tannins form complexes with digestive enzymes, proteins and starch and reduces their nutritive val
Reagent: 2% of tannic acid. Procedure: Remove sample to watch glass or microscope slide, first wet, and then heat to induce extract. Remove the slide from heat and add a drop of tannic acid to the sample extract. Results: Appearance of Dirty-white precipitate indicates the presence of gelatin. Interference: Starch can interfere with the formation of precipitate (Ebnesajjad et al,2010).
Fig 13: Tannin test for detecting presence of glue
Various solid wastes are recovered from the tannery effluent such as glue, gelatin, animal feed manufacture, fuel, fertilizer and soil conditioner etc. Among these glue and gelatin can be produced from several Tannery waste byproducts. During leather production the vital waste that is left prior to tanning and after the limed hides has been split off is the base layer of the skin along with the attached fatty acid tissue. The main characteristics of this proteinaceous layer include a very high water content of up to 870 g kg−1 and a fair content of protein of about 40–60 g kg−1of the dry mass, carbohydrates and the fat content being 10–20 g kg−1 of the dry mass(Banerjee et al,2007). The proposed treatment involves:
Washing of the solid tannery waste to remove the inorganic salts
Fat—cattle tallow (4–12% of the total mass of the initial material)
Collagen hydrosylate—glue (5–10%)
Proteins concentrate for fodder (1–3% yield)
Hydrolysis with alkaline proteinase for additional extraction of protein from the insoluble residue
Fat separation and collagen extraction in hot water solution
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